First report of the morphological and molecular characterization of Pseudolynchia canariensis (Diptera: Hippoboscidae) from Al-Baha region, Saudi Arabia.

This study aims to study the morphological and molecular characterization of (Pseudolynchia canariensis; Macquart, 1839)in the Al-Baha region of Saudi Arabia. Ninety-four pigeons were obtained from traditional pigeon breeding farms of the Al-Baha region, and fly samples were collected. Taxonomic keys were used to define the morphology of flies, whereas molecular characteristics were identified based on cytochrome c oxidase subunit 1. The rate of Pseudolynchia canariensis infestation in the examined pigeons was 44.5%. The genetic sequences of the fly samples were deposited in GenBank (accession number OQ073507). The match rate between the fly samples from the present study and those previously recorded in GenBank (accession numbers: EF531220, OM073981, and MW853922) displayed 99.66%. This study demonstrates that Pseudolynchia canariensis is common in the Al-Baha region; thus, further studies are required to detect other species from the same genus and their geographical distribution.

Rosemary and neem methanolic extract: antioxidant, cytotoxic, and larvicidal activities supported by chemical composition and molecular docking simulations.

This study aimed to employ GC-MS to assess the chemical composition of MeOH leaf extracts of R. officinalis and A. indica and evaluate their insecticidal, antioxidant, and antibacterial activities. Twelve components, representing 98.61% and 100% of the total volatile compounds, were deduced from the extracted R. officinalis and A. indica, respectively, using this method. In R. officinalis extract, limonene is typically positioned as the main component (23.03%), while the main chemicals identified in A. indica extract were methyl (E)-octadec-13-enoate (23.20%) and (2R)-1,3,8-trimethyl-4-propyl-5-ethyl-2-(1-hydroxyethyl)-7-methoxycardonylethyl-6-methylenecarbonyl-porphyrin (23.03%). Both extracts of R. officinalis and A. indica exhibited different toxicity against the stored grain pest T. castaneum, with LC50 values of 1.470 and 2.588 mg/ml, respectively. Additionally, after 4 and 5 h of treatment at a concentration of 0.2 mg/ml, the A. indica extract showed the highest levels of repellent action (81.4% and 93.4%), and the R. officinalis extract showed a good repellent rate (64.9% and 80.7%) against T. castenum larvae. With an IC50 value of 35.83 and 28.68 mg/L and a radical scavenging activity percentage of 67.76% and 72.35%, the leaf extract was found to be the most potent plant extract when tested for DPPH antioxidant activity. Overall results showed that MeOH extracts of R. officinalis and A. indica were more effective against S. aureus than E. coli. To determine how the investigated chemicals attach to the active sites of E. coli DNA gyrase A and S. aureus undecaprenyl diphosphate synthase, docking studies were carried out. The consensus score analysis showed that limonene exhibits the best binding energy with both enzymes in docking analysis and more stability in molecular dynamics simulations. The RMSD was obtained at 20.6 and 4.199 (Kcal/mole). The two compounds were successfully used in molecular dynamics simulation research to generate stable complexes with DNA gyrase A.